mab mip8a Search Results


anti-fc ri mab mip8a  (Bio-Rad)
90
Bio-Rad anti-fc ri mab mip8a
Anti Fc Ri Mab Mip8a, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-fc ri mab mip8a/product/Bio-Rad
Average 90 stars, based on 1 article reviews
anti-fc ri mab mip8a - by Bioz Stars, 2026-04
90/100 stars
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anti human cd89 monoclonal antibody  (Bio-Rad)
93
Bio-Rad anti human cd89 monoclonal antibody
Cytotoxic recruitment of <t>CD89+</t> effector cells by CD20-IgA. (A-C) The ability of anti-CD20 antibodies to mediate human leukocyte recruitment on lymphoma target cells was assessed using the ImageStream® Imaging Flow Cytometer. CFSE-labeled DHL-4 cells were pre-incubated with 20 μg/mL of each CD20 antibody, mixed with human leukocytes and stained with anti-human CD16 PE, anti-human CD45 ECD and DRAQ5 as a nuclear marker. Analyses were performed using the Amnis IDEAS software to evaluate the percentage of CFSE+ targets associated with CD16+ effector leukocytes. (A) Fluorescence and bright field images of representative CFSE+ cells alone or in aggregates with leukocytes. (BF; bright field) (B) Percentage of DHL-4 cells associated with one or more CD16+ effector leukocytes. Asterisks indicate statistically significant differences with a paired t test **P<0.01. (C) Inhibition of mIgA2-mediated target/CD16+ effector aggregation in the presence of antihuman CD89 antibodies. (D) ADCC induced by mIgA2 in human CD89-transgenic or wild-type (WT) mice as evaluated by IVAK; 106 mixed labeled target (EL4-CD20) and reference (EL4 WT) cells were injected i.p. before injection of 20 μg anti-CD20 IgG1, mIgA2 or control. Five hours later, hCD20 -specific killing was calculated from ratios of target to reference cells in the peritoneal washings from individual mice. Data are representative of at least six mice for each condition (asterisks indicate statistically significant differences between values observed for transgenic mice compared to WT mice with an unpaired t test **P<0.01).
Anti Human Cd89 Monoclonal Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human cd89 monoclonal antibody/product/Bio-Rad
Average 93 stars, based on 1 article reviews
anti human cd89 monoclonal antibody - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
anti human fc ri mab mip8a migg1  (Bio-Rad)
94
Bio-Rad anti human fc ri mab mip8a migg1
Cytotoxic recruitment of <t>CD89+</t> effector cells by CD20-IgA. (A-C) The ability of anti-CD20 antibodies to mediate human leukocyte recruitment on lymphoma target cells was assessed using the ImageStream® Imaging Flow Cytometer. CFSE-labeled DHL-4 cells were pre-incubated with 20 μg/mL of each CD20 antibody, mixed with human leukocytes and stained with anti-human CD16 PE, anti-human CD45 ECD and DRAQ5 as a nuclear marker. Analyses were performed using the Amnis IDEAS software to evaluate the percentage of CFSE+ targets associated with CD16+ effector leukocytes. (A) Fluorescence and bright field images of representative CFSE+ cells alone or in aggregates with leukocytes. (BF; bright field) (B) Percentage of DHL-4 cells associated with one or more CD16+ effector leukocytes. Asterisks indicate statistically significant differences with a paired t test **P<0.01. (C) Inhibition of mIgA2-mediated target/CD16+ effector aggregation in the presence of antihuman CD89 antibodies. (D) ADCC induced by mIgA2 in human CD89-transgenic or wild-type (WT) mice as evaluated by IVAK; 106 mixed labeled target (EL4-CD20) and reference (EL4 WT) cells were injected i.p. before injection of 20 μg anti-CD20 IgG1, mIgA2 or control. Five hours later, hCD20 -specific killing was calculated from ratios of target to reference cells in the peritoneal washings from individual mice. Data are representative of at least six mice for each condition (asterisks indicate statistically significant differences between values observed for transgenic mice compared to WT mice with an unpaired t test **P<0.01).
Anti Human Fc Ri Mab Mip8a Migg1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human fc ri mab mip8a migg1/product/Bio-Rad
Average 94 stars, based on 1 article reviews
anti human fc ri mab mip8a migg1 - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier

Image Search Results


Cytotoxic recruitment of CD89+ effector cells by CD20-IgA. (A-C) The ability of anti-CD20 antibodies to mediate human leukocyte recruitment on lymphoma target cells was assessed using the ImageStream® Imaging Flow Cytometer. CFSE-labeled DHL-4 cells were pre-incubated with 20 μg/mL of each CD20 antibody, mixed with human leukocytes and stained with anti-human CD16 PE, anti-human CD45 ECD and DRAQ5 as a nuclear marker. Analyses were performed using the Amnis IDEAS software to evaluate the percentage of CFSE+ targets associated with CD16+ effector leukocytes. (A) Fluorescence and bright field images of representative CFSE+ cells alone or in aggregates with leukocytes. (BF; bright field) (B) Percentage of DHL-4 cells associated with one or more CD16+ effector leukocytes. Asterisks indicate statistically significant differences with a paired t test **P<0.01. (C) Inhibition of mIgA2-mediated target/CD16+ effector aggregation in the presence of antihuman CD89 antibodies. (D) ADCC induced by mIgA2 in human CD89-transgenic or wild-type (WT) mice as evaluated by IVAK; 106 mixed labeled target (EL4-CD20) and reference (EL4 WT) cells were injected i.p. before injection of 20 μg anti-CD20 IgG1, mIgA2 or control. Five hours later, hCD20 -specific killing was calculated from ratios of target to reference cells in the peritoneal washings from individual mice. Data are representative of at least six mice for each condition (asterisks indicate statistically significant differences between values observed for transgenic mice compared to WT mice with an unpaired t test **P<0.01).

Journal: Haematologica

Article Title: Anti-CD20 IgA can protect mice against lymphoma development: evaluation of the direct impact of IgA and cytotoxic effector recruitment on CD20 target cells

doi: 10.3324/haematol.2011.061408

Figure Lengend Snippet: Cytotoxic recruitment of CD89+ effector cells by CD20-IgA. (A-C) The ability of anti-CD20 antibodies to mediate human leukocyte recruitment on lymphoma target cells was assessed using the ImageStream® Imaging Flow Cytometer. CFSE-labeled DHL-4 cells were pre-incubated with 20 μg/mL of each CD20 antibody, mixed with human leukocytes and stained with anti-human CD16 PE, anti-human CD45 ECD and DRAQ5 as a nuclear marker. Analyses were performed using the Amnis IDEAS software to evaluate the percentage of CFSE+ targets associated with CD16+ effector leukocytes. (A) Fluorescence and bright field images of representative CFSE+ cells alone or in aggregates with leukocytes. (BF; bright field) (B) Percentage of DHL-4 cells associated with one or more CD16+ effector leukocytes. Asterisks indicate statistically significant differences with a paired t test **P<0.01. (C) Inhibition of mIgA2-mediated target/CD16+ effector aggregation in the presence of antihuman CD89 antibodies. (D) ADCC induced by mIgA2 in human CD89-transgenic or wild-type (WT) mice as evaluated by IVAK; 106 mixed labeled target (EL4-CD20) and reference (EL4 WT) cells were injected i.p. before injection of 20 μg anti-CD20 IgG1, mIgA2 or control. Five hours later, hCD20 -specific killing was calculated from ratios of target to reference cells in the peritoneal washings from individual mice. Data are representative of at least six mice for each condition (asterisks indicate statistically significant differences between values observed for transgenic mice compared to WT mice with an unpaired t test **P<0.01).

Article Snippet: For inhibition assays, leukocytes were pre-incubated with 1 ng to 10 μg/mL of an anti-human CD89 monoclonal antibody (clone MIP8a, AbD serotec).

Techniques: Imaging, Flow Cytometry, Labeling, Incubation, Staining, Marker, Software, Fluorescence, Inhibition, Transgenic Assay, Injection, Control